Laboratory Exercises in Microbiology – Lansing M. Prescott, John P. Harley – 5th Edition


Laboratory Exercises in Microbiology, 5/e has been prepared to accompany Prescott et al’s Microbiology, 5/e. Like the text, the laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology.

Prescott, Harley and Klein’s 5th edition provides a balanced, comprehensive introduction to all major areas of microbiology. Because of this balance, Microbiology, is appropriate for students preparing for careers in medicine, dentistry, nursing, and allied health, as well as research, teaching, and industry. Biology and chemistry are prerequisites.

Prescott’s contains briefer chapters than most books, but more of them (42). Students will find the concise chapters more palatable and less intimidating. Short chapters give the instructor the opportunity to fit the text more closely to the instructor’s syllabus. Topic flexibility is allowed.

There is an outstanding pedagogical including outlines, concepts, key terms, cross-referencing, readings, new critical thinking questions, etc., to help students understand difficult material.

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  • 1 Microscopic Techniques
    1 Bright-Field Light Microscope and Microscopic Measurement of Organisms
    2 The Hanging Drop Slide and Bacterial Motility
    3 Dark-Field Light Microscope
    4 Phase-Contrast Light Microscope
    5 Flourescence Microscope
    2 Bacterial Morphology and Staining
    6 Negative Staining
    7 Smear Preparation and Simple Staining
    8 Gram Stain
    9 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
    10 Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
    11 Capsule Staining
    12 Flagella Staining: West and Difco's SpotTest Methods
    3 Basic Laboratory and Culture Techniques
    13 Microbiological Culture Media Preparation and Sterilization
    14 Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
    15 Spread-Plate Technique
    16 The Streak-Plate Technique and Different Media
    17 Pour-Plate Technique
    18 Cultivation of Anaerobic Bacteria
    19 Determination of Bacterial Numbers
    4 Biochemical Activities of Bacteria
    20 Carbohydrates I: Fermentation and B-Galactosidase Activity
    21 Carbohydrates II: Triple Sugar Iron Agar Tests
    22 Carbohydrates III: Starch Hydrolysis
    23 Lipids: Lipid Hydrolysis
    24 Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
    25 Proteins, Amino Acids, and Enzymes II: The IMViC Tests
    26 Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
    27 Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
    28 Proteins, Amino Acids, and Enzymes V: Catalase Activity
    29 Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
    30 Proteins, Amino Acids, and Enzymes VII:Oxidase Test
    31 Proteins, Amino Acids, and Enzymes VIII: Urease Activity
    32 Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test
    33 Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
    34 Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
    5 Rapid Multitest Systems
    35 The API 20E System
    36 The Enterotube II System
    6 Unknown Identification
    37 Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria
    38 General Unknown
    7 Environmental Factors Affecting Growth of Microorganisms
    39 Temperature
    40 pH
    41 Osmotic Pressure
    42 The Effects of Chemical Agents on Bacteria I: Disinfectants
    43 The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
    44 Hand Washing, Environmental Sampling, and Microbiological Monitoring
    45 Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
    8 Environmental and Food Microbiology
    46 Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test
    47 Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms
    48 Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
    49 Enumeration of Soil Microorganisms
    50 Bacterial Count of a Food Product
    51 Examination of Milk for Bacteria
    9 Medical Microbiology
    52 Agglutination Reactions: Blood Groups
    53 Isolation of Normal Microbiota from the Human Body
    54 Staphylococci
    55 Pneumococci
    56 Streptococci
    57 Neisseriae
    58 Aerobic and Anaerobic Endospore-Forming Bacteria
    10 Survey of Selected Eucaryotic Microorganisms
    59 Fungi I: Yeasts
    60 Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes
    11 Microbial Genetics and Genomics
    61 Bacterial Mutation
    62 Bacterial Transformation
    63 Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
    64 Isolation of Genomic DNA from Saccharomyces cerevisiae
    65 Isolation and Purification of Genomic DNA from Escherichia coli
    66 Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis

    Appendix A Dilutions with Sample Problems
    Appendix B Metric and English Measurement Equivalents
    Appendix C Transmission-Absorbance Table for Spectrophotometry
    Appendix D Logarithms
    Appendix E pH and pH Indicators
    Appendix F Scientific Notation
    Appendix G Identification Charts
    Appendix H Reagents, Solutions, Stains, and Tests
    Appendix I Culture Media
    Appendix J Sources and Maintenance of Microbiological Stock Cultures
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