Laboratory Exercises in Microbiology – Lansing M. Prescott, John P. Harley – 5th Edition


Laboratory Exercises in Microbiology, 5/e has been prepared to accompany Prescott et al’s Microbiology, 5/e. Like the text, the laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology.

Prescott, Harley and Klein’s 5th edition provides a balanced, comprehensive introduction to all major areas of microbiology. Because of this balance, Microbiology, is appropriate for students preparing for careers in medicine, dentistry, nursing, and allied health, as well as research, teaching, and industry. and are prerequisites.

Prescott’s contains briefer chapters than most books, but more of them (42). Students will find the concise chapters more palatable and less intimidating. Short chapters give the instructor the opportunity to fit the text more closely to the instructor’s syllabus. Topic flexibility is allowed.

There is an outstanding pedagogical including outlines, concepts, key terms, cross-referencing, readings, new critical thinking questions, etc., to help students understand difficult material.

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Table of Contents

1 Microscopic Techniques
1 Bright-Field Light Microscope and Microscopic Measurement of Organisms
2 The Hanging Drop Slide and Bacterial Motility
3 Dark-Field Light Microscope
4 Phase-Contrast Light Microscope
5 Flourescence Microscope
2 Bacterial Morphology and Staining
6 Negative Staining
7 Smear Preparation and Simple Staining
8 Gram Stain
9 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
10 Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
11 Capsule Staining
12 Flagella Staining: West and Difco's SpotTest Methods
3 Basic Laboratory and Culture Techniques
13 Microbiological Culture Media Preparation and Sterilization
14 Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
15 Spread-Plate Technique
16 The Streak-Plate Technique and Different Media
17 Pour-Plate Technique
18 Cultivation of Anaerobic Bacteria
19 Determination of Bacterial Numbers
4 Biochemical Activities of Bacteria
20 Carbohydrates I: Fermentation and B-Galactosidase Activity
21 Carbohydrates II: Triple Sugar Iron Agar Tests
22 Carbohydrates III: Starch Hydrolysis
23 Lipids: Lipid Hydrolysis
24 Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
25 Proteins, Amino Acids, and Enzymes II: The IMViC Tests
26 Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
27 Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
28 Proteins, Amino Acids, and Enzymes V: Catalase Activity
29 Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
30 Proteins, Amino Acids, and Enzymes VII:Oxidase Test
31 Proteins, Amino Acids, and Enzymes VIII: Urease Activity
32 Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test
33 Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
34 Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
5 Rapid Multitest Systems
35 The API 20E System
36 The Enterotube II System
6 Unknown Identification
37 Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria
38 General Unknown
7 Environmental Factors Affecting Growth of Microorganisms
39 Temperature
40 pH
41 Osmotic Pressure
42 The Effects of Chemical Agents on Bacteria I: Disinfectants
43 The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
44 Hand Washing, Environmental Sampling, and Microbiological Monitoring
45 Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
8 Environmental and Food Microbiology
46 Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test
47 Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms
48 Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
49 Enumeration of Soil Microorganisms
50 Bacterial Count of a Food Product
51 Examination of Milk for Bacteria
9 Medical Microbiology
52 Agglutination Reactions: Blood Groups
53 Isolation of Normal Microbiota from the Human Body
54 Staphylococci
55 Pneumococci
56 Streptococci
57 Neisseriae
58 Aerobic and Anaerobic Endospore-Forming Bacteria
10 Survey of Selected Eucaryotic Microorganisms
59 Fungi I: Yeasts
60 Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes
11 Microbial Genetics and Genomics
61 Bacterial Mutation
62 Bacterial Transformation
63 Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
64 Isolation of Genomic DNA from Saccharomyces cerevisiae
65 Isolation and Purification of Genomic DNA from Escherichia coli
66 Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis

Appendix A Dilutions with Sample Problems
Appendix B Metric and English Measurement Equivalents
Appendix C Transmission-Absorbance Table for Spectrophotometry
Appendix D Logarithms
Appendix E pH and pH Indicators
Appendix F Scientific Notation
Appendix G Identification Charts
Appendix H Reagents, Solutions, Stains, and Tests
Appendix I Culture Media
Appendix J Sources and Maintenance of Microbiological Stock Cultures

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